Source: Annual Congress 2007 - Noninvasive evaluation of airway diseases
Disease area: Airway diseases

Abstract

Clara cell secretory protein (CC16) is secreted by Clara cells and has antiinflammatory properties within the lung. It seems to be a marker for airway epithelial injury. While increased levels of CC16 were found in broncho-alveolar lavage fluid (BALF) of patients with acute respiratory distress syndrome, reduced levels of CC 16 have been found in BALF of smokers, patients with lung cancer, COPD or asthma. The aim of the study presented was to determine whether CC16 can be measured in exhaled breath condensate and whether levels of CC16 differentiate between asthmatics and healthy controls.
Breath condensate was sampled from 15 healthy adults during 10 min of relaxed tidal breathing with a commercially available condenser (EcoScreen, JAEGER). CC16 was analysed with an ELISA (BioVendor Laboratory Medicine, Inc.) before and after lyophilisation of the sample. Further the recovery rate of the positive control within the breath condensate was analysed.
CC16 was neither detectable in the native nor in the lyophilized samples. The recovery rate of CC16 positive controls within exhaled breath condensate was good (min. 4,39 ng/ml).
The present study results suggest that exhaled breath condensate analysis is not a suitable method for detection of CC16. One reason for this might be because of concentrations below detection limit of CC16 within the condensate. This again might be due to the peripheral localisation of the clara cells within the airways.

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Citations should be made in the following way:
C. Vogelberg, S. Ruß, K. Marx, A. Böhm, A. Rösen-Wolff (Dresden, Germany). Clara cell secretory protein (CC16): possibility of detection in exhaled breath condensate. Eur Respir J 2007; 30: Suppl. 51, 528

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